The study utilizes a large collection of fluorescently marked Ds-GFP insertional mutations in haploid maize pollen to link gene disruptions with quantitative fitness effects measured as transmission deviations. By integrating genome-derived features (e.g., codon usage) and expression profiling into interpretable machine learning models, they achieve high predictive performance (auROC >90%) for genes influencing pollen fitness, highlighting expression specificity as a key predictor.

The study examined how polyploidy, hybridization, and incomplete lineage sorting affect phylogenetic reconstructions in the genus Packera, evaluating several published paralog‑processing pipelines. Results showed that the choice of orthology and paralog handling methods markedly altered tree topology, time‑calibrated phylogenies, biogeographic histories, and detection of ancient reticulation, underscoring the need for careful methodological selection alongside comprehensive taxon sampling.

The study utilizes explainable artificial intelligence (XAI) combined with machine learning to assess how inter‑annual weather variability influences oilseed sunflower yields across the United States from 1976 to 2022. Key climate predictors, especially summer maximum temperature and total precipitation, were identified, and predictive models were projected under various Shared Socioeconomic Pathways to 2080, revealing region‑specific yield declines.