Phylogenomic challenges in polyploid-rich lineages: Insights from paralog processing and reticulation methods using the complex genus Packera (Asteraceae: Senecioneae)
Authors: Moore-Pollard, E. R., Ellestad, P., Mandel, J.
The study examined how polyploidy, hybridization, and incomplete lineage sorting affect phylogenetic reconstructions in the genus Packera, evaluating several published paralog‑processing pipelines. Results showed that the choice of orthology and paralog handling methods markedly altered tree topology, time‑calibrated phylogenies, biogeographic histories, and detection of ancient reticulation, underscoring the need for careful methodological selection alongside comprehensive taxon sampling.
High radiosensitivity in the conifer Norway spruce (Picea abies) due to lesscomprehensive mobilisation of protection and repair responses compared to the radiotolerant Arabidopsis thaliana
Authors: Bhattacharjee, P., Blagojevic, D., Lee, Y., Gillard, G. B., Gronvold, L., Hvidsten, T. R., Sandve, S. R., Lind, O. C., Salbu, B., Brede, D. A., Olsen, J. E.
The study compared early protective, repair, and stress responses to chronic gamma irradiation in the radiosensitive conifer Norway spruce (Picea abies) and the radiotolerant Arabidopsis thaliana. Norway spruce exhibited growth inhibition, mitochondrial damage, and higher DNA damage at low dose rates, while Arabidopsis maintained growth, showed minimal organelle damage, and activated DNA repair and antioxidant genes even at the lowest dose rates. Transcriptomic analysis revealed that the tolerant species mounts a robust transcriptional response at low doses, whereas the sensitive species only responds at much higher doses.