The study examined how dual‑purpose hemp (Cannabis sativa) adjusts to different phosphate levels, showing that flower biomass is maintained unless phosphate is completely removed. Integrated physiological measurements and transcriptomic profiling revealed that phosphate is reallocated to flowers via glycolytic bypasses and organic phosphate release, while key regulatory genes followed expected patterns but did not suppress uptake at high phosphate, leading to nitrate depletion that limits growth.
The study identified a heat‑responsive exon‑skipping event in the basic Helix‑Loop‑Helix domain of the transcription factor PIF4, which reduces PIF4 activity and promotes photomorphogenic traits in etiolated seedlings. This reveals a novel post‑transcriptional mechanism by which plants modulate PIF4 function during heat stress.
The study evaluated how stomatal anatomy and physiological efficiency influence wheat heat tolerance across multi‑environment field trials with 200 genotypes, using early versus delayed sowing to impose temperature stress. Findings revealed a decoupling between anatomical capacity (gsmax) and actual conductance (gs, gse) under heat, plastic shifts toward smaller, denser stomata, and identified 125 QTL linked to stomatal traits, suggesting targets for breeding climate‑resilient wheat.
The study identified a critical two‑week window of elevated maternal temperature during weeks 4–5 after flowering that delays dormancy release in weedy rice seeds. Controlled‑environment and field transplant experiments showed that this late‑reproductive‑stage heat exposure postpones germination after after‑ripening, providing insight for predicting seed behavior and improving weed management strategies.
Using ten Phaeodactylum tricornutum mutant strains with graded constitutive Lhcx1 expression, the study links NPQ induction under high light to physiological outcomes (oxidized QA, increased cyclic electron flow) and extensive transcriptomic reprogramming, affecting nearly half the genome. The approach demonstrates that higher NPQ mitigates PSII damage, boosts ATP production for repair, and drives distinct gene regulatory networks, providing a model framework for dissecting photosynthetic and gene expression integration.
The study establishes a tractable system using the large bloom-forming diatom Coscinodiscus granii and its natural oomycete parasite Lagenisma coscinodisci, enabling manual isolation of single host cells and stable co-cultures. High‑quality transcriptomes for both partners were assembled, revealing diverse oomycete effectors and a host transcriptional response involving proteases and exosome pathways, while also profiling the co‑occurring heterotrophic flagellate Pteridomonas sp. This tripartite platform provides a unique marine model for dissecting molecular mechanisms of oomycete‑diatom interactions.
Using a forward genetic screen of 284 Arabidopsis thaliana accessions, the study identified extensive natural variation in root endodermal suberin and pinpointed the previously unknown gene SUBER GENE1 (SBG1) as a key regulator. GWAS and protein interaction analyses revealed that SBG1 controls suberin deposition by binding type‑one protein phosphatases (TOPPs), with disruption of this interaction or TOPP loss‑of‑function altering suberin levels, linking the pathway to ABA signaling.
Identification of a putative RBOHD-FERONIA-CRK10-PIP2;6 plasma membrane complex that interacts with phyB to regulate ROS production in Arabidopsis thaliana
Authors: Mohanty, D., Fichman, Y., Pelaez-Vico, M. A., Myers, R. J., Sealander, M., Sinha, R., Morrow, J., Eckstein, R., Olson, K., Xu, C., An, H., Yoo, C. Y., Zhu, J.-K., Zhao, C., Zandalinas, S. I., Liscum, E., Mittler, R.
The study demonstrates that FERONIA and phytochrome B physically interact with the NADPH oxidase RBOHD, and that FERONIA-mediated phosphorylation of phyB is essential for RBOHD-driven ROS production under excess light stress in Arabidopsis thaliana. Additional membrane proteins CRK10 and PIP2;6 also associate with this complex, forming a plasma‑membrane assembly that integrates multiple signaling pathways to regulate stress‑induced ROS.
The mRNA covalent modification dihydrouridine regulates transcript turnover and photosynthetic capacity during plant abiotic stress
Authors: Yu, L., Melandri, G., Dittrich, A. C., Calleja, S., Rozzi, B., Ganguly, D. R., Palos, K., Srinivasan, A., Brewer, E. K., Fischer, H., Obata, T., Elgawad, H. A., Beemster, G. T. S., Henderson, R., Garcia, C. D., Zhang, X., Stern, D., Eveland, A., Schroeder, S. J., Skirycz, A., Lyons, E., Arnold, E. A., Gregory, B. D., Nelson, A. D. L., Pauli, D.
The study integrates multi-omics data from six Sorghum bicolor accessions under field drought to link RNA covalent modifications (RCMs) with photosynthetic performance, identifying the enzyme SbDUS2 that produces dihydrouridine (DHU) on transcripts. Loss‑of‑function dus2 mutants in Arabidopsis thaliana reveal that DHU deficiency leads to hyperstability of photosynthesis‑related mRNAs, impairing germination, development, and stress‑induced CO2 assimilation. The authors propose DHU as a post‑transcriptional mark that promotes rapid mRNA turnover during abiotic stress, enhancing plant resilience.
Proline transporters balance the salicylic acid-mediated trade-off between regeneration and immunity in plants
Authors: Yang, L., Xu, D., Belew, Z. M., Cassia Ferreira Dias, N., Wang, L., Zhang, A., Chen, Y.-F. S., Newton, C. J., Kong, F., Zheng, Y., Yao, Y., Brewer, M. T., Teixeira, P. J. P. L., Nour-Eldin, H. H., Xu, D.
The study identifies wound‑induced proline transporters ProT2 and ProT3 as central regulators that link salicylic acid signaling to the suppression of de novo root regeneration (DNRR) via modulation of reactive oxygen species dynamics. Genetic loss of these transporters or pharmacological inhibition of proline transport alleviates SA‑mediated regeneration inhibition across several plant species without compromising disease resistance.