Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes
Authors: Frances, N., Giustini, C., Finazzi, G., Ferro, M., Albanese, P.
Date: 2025-10-08 · Version: 1
DOI: 10.1101/2025.10.07.681025 Category: Plant Biology
Model Organism: Multi-species
▶ AI Summary
The study introduces an enhanced crosslinking mass spectrometry workflow that preserves native protein interactions within functional thylakoid membranes of Arabidopsis and spinach, while electron transport remains active. Mapping the obtained crosslinks to known structures validates complex integrity and reveals novel assemblies, facilitating in situ exploration of photosynthetic membrane protein networks.
photosynthesis thylakoid membranes crosslinking mass spectrometry protein complexes Arabidopsis thaliana