Researchers isolated a fungal pathogen from a naturally infected Rumex crispus leaf in Japan and identified it as Teratoramularia rumicicola using morphological traits and phylogenetic analysis of ITS and LSU rDNA sequences. Host range tests showed the isolate (TR4) caused disease and reduced biomass in three Rumex species but was harmless to five tested forage crops, indicating its potential as a selective bioherbicide for pasture systems.

Large-scale bioinformatics identified a new class of transmembrane phosphotransfer proteins (TM‑HPt) across 61 plant species, showing conserved HPt motifs and potential activity in multistep phosphorelay signaling. Phylogenetic relationships were inferred via Bayesian DNA analysis, expression was validated by transcriptomics, and molecular modeling suggested possible membrane-associated structural arrangements.

The study identifies a novel C-terminal FR motif in Lotus japonicus NODULE INCEPTION (NIN) that expands DNA‑binding specificity by stabilizing the RWP‑RK dimer, and shows that loss of this motif impairs nodulation and nitrogen fixation. Comparative analysis reveals that Arabidopsis NLP2 also possesses a NIN‑type FR, and phylogenetic data suggest the motif originated in early gymnosperms, indicating it predates the evolution of root nodule symbiosis.

The study reconstructed the evolutionary history of plant-specific GBF1-type ARF-GEFs by building phylogenetic trees and ortho‑synteny groups, identifying orthologs of AtGNOM and AtGNL1 across species. Functional analyses using transgenic Arabidopsis lines and yeast two‑hybrid assays revealed how duplication and loss events diversified GNOM paralogs, separating polar recycling from secretory trafficking functions.

The study investigates the function of two GT47B arabinan arabinosyltransferases in the liverwort Marchantia polymorpha, generating loss‑of‑function and overexpression lines to assess cell wall composition. Using CoMPP, glycosyl linkage analysis, and LM6 immunolabelling, the authors found that MpARADL2 mutants have reduced 1,5‑L‑arabinan epitopes in elaters despite unchanged overall 5‑linked Araf levels, suggesting additional enzymes compensate in thallus tissue. Attempts to express and purify the enzymes in HEK293 cells failed, implying a clade‑specific solubility requirement and highlighting the need to identify interacting partners.

The study characterizes the composition and structure of cell wall glycans in eight tissue types of the liverwort Marchantia polymorpha, revealing both typical land‑plant features and unique traits such as abundant (1,5)-arabinan in sporophytes and low overall pectin levels. Comparative genomic analysis shows a diversified glycosyltransferase repertoire relative to Arabidopsis, and the authors created a Gateway‑compatible library of 93 M. polymorpha GTs to facilitate future functional studies.

The authors identified MpCAFA, a protein combining CAPS-like and FAP115-like domains, as a key factor for rapid ciliary swimming in the liverwort Marchantia polymorpha spermatozoids. Loss-of-function mutants displayed markedly reduced swimming speed despite normal axoneme structure, chemotaxis, and fertility, and these defects were rescued by a MpCAFA‑mCitrine fusion that localized along the entire cilium. Both the CAPS-like and FAP115-like regions are required for MpCAFA’s function and ciliary targeting, establishing it as a major ciliary protein and a marker for visualizing spermatozoid motility.

The study investigated meristem activation in the liverwort Marchantia polymorpha, revealing that simulated shade causes alternating inactivity of meristems. Transcriptomic comparison of active versus inactive meristems identified the cytochrome P450 monooxygenase MpCYP78E1 as an inhibitor of meristem activity and initiation, with loss- and gain-of-function mutants confirming its regulatory role in shoot branching architecture.

The study demonstrates that MYB‑bHLH‑WDR transcriptional complexes (MBW) are present in the liverwort Marchantia polymorpha, indicating that such complexes originated before the diversification of land plants. Functional analyses reveal that two MYB paralogs, MpMYB14 and MpMYB02, rely on a single bHLH partner (MpbHLH12) to regulate flavonoid biosynthesis and liverwort‑specific oil body maturation, suggesting an ancestral role in pigment production and a derived role in organelle development.

The authors adapted OpenPlant kit CRISPR/Cas9 tools to enable multiplex gRNA expression from a single transcript using tRNA sequences in the liverwort Marchantia polymorpha, markedly enhancing editing efficiency and scalability. They coupled this vector system with a simplified, optimized thallus transformation protocol, providing a rapid and versatile platform for generating CRISPR/Cas9 mutants and advancing functional genomics in this model species.