The study identified lineage-specific long non‑coding RNAs (lncRNAs) from the aphid‑specific Ya gene family in Rhopalosiphum maidis and R. padi, demonstrating that these Ya lncRNAs are secreted into maize, remain stable, and move systemically. RNA interference of Ya genes reduced aphid fecundity, while ectopic expression of Ya lncRNAs in maize enhanced aphid colonization, indicating that Ya lncRNAs act as cross‑kingdom effectors that influence aphid virulence.

The study provides a comprehensive genome-wide catalog and single‑cell expression atlas of the carbonic anhydrase (CA) gene family in maize, identifying 18 CA genes across α, β, and γ subfamilies and detailing their structural and regulatory features. Phylogenetic, synteny, promoter motif, bulk tissue RNA‑seq, and single‑cell RNA‑seq analyses reveal distinct tissue and cell‑type specific expression patterns, highlighting β‑CAs as key players in C4 photosynthesis and γ‑CAs in ion/pH buffering, and propose cell‑type‑specific CA genes as targets for improving stress resilience.

The study used a computer‑vision phenotyping pipeline (EarVision.v2) based on Faster R-CNN to map Ds‑GFP mutant kernels on maize ears and a statistical framework (EarScape) to assess spatial patterns of allele transmission from the apex to the base. They found that alleles causing pollen‑specific transmission defects often show significant spatial biases, whereas Mendelian alleles do not, indicating that reduced pollen fitness can shape the spatial distribution of progeny genotypes in Zea mays.

The study presents a plant‑focused phylogenetic analysis of class B flavin‑dependent monooxygenases, identifying eight distinct families and revealing lineage‑specific diversification, especially in the NADPH‑binding domain. Using known FMOs as baits, they assembled flavin‑related proteins from key Viridiplantae lineages, performed domain architecture and motif analyses, and reclassified several families, providing a framework for future functional studies.

Molecular phylogenetic analysis indicated that diatom Lhcx proteins share a common ancestor with green algal Lhcsrs, suggesting acquisition via horizontal gene transfer. Knockout of the Lhcx1 gene in the diatom Chaetoceros gracilis almost eliminated non‑photochemical quenching and revealed that Lhcx1 mediates quenching in detached antenna complexes, while also influencing PSII quantum yield and carbon fixation under high‑light conditions. These findings elucidate the evolutionary origin and mechanistic role of Lhcx‑mediated photoprotection in diatoms.

The study employed computational approaches to characterize the SUMOylation (ULP) machinery in Asian rice (Oryza sativa), analyzing phylogenetic relationships, transcriptional patterns, and protein structures across the reference genome, a population panel, and wild relatives. Findings reveal an expansion of ULP genes in cultivated rice, suggesting selection pressure during breeding and implicating specific ULPs in biotic and abiotic stress responses, providing resources for rice improvement.

The study investigates how the pleiotropic maize genes GRASSY TILLERS1 (GT1) and RAMOSA3 (RA3) are differentially regulated to suppress axillary meristems and floral organs, using a newly developed high-throughput quantitative phenotyping method for grass flowers. Distinct environmental mechanisms were found to control each suppression process, and upstream regulatory pathways of GT1 and RA3 have diverged, illustrating how ancient developmental genes can be redeployed to increase genetic pleiotropy during evolution.

The study reveals that the maize catalytic trehalose-6-phosphate phosphatase RA3 interacts with the non‑catalytic TPS ZmTPS1, and together with the catalytic TPS ZmTPS14 they form a protein complex that enhances enzymatic activity. Genetic analyses show that mutations in ZmTPS1 and its paralog ZmTPS12 exacerbate ra3 branching phenotypes, while loss of the catalytic TPSs ZmTPS11 and ZmTPS14 causes embryonic lethality, indicating essential and regulatory roles for both catalytic and non‑catalytic TPS/TPP proteins in plant development.

Researchers isolated a fungal pathogen from a naturally infected Rumex crispus leaf in Japan and identified it as Teratoramularia rumicicola using morphological traits and phylogenetic analysis of ITS and LSU rDNA sequences. Host range tests showed the isolate (TR4) caused disease and reduced biomass in three Rumex species but was harmless to five tested forage crops, indicating its potential as a selective bioherbicide for pasture systems.

Using CRISPR‑Cas9‑generated Zmcry mutants, the study shows that maize cryptochromes redundantly mediate blue‑light signaling, suppress mesocotyl elongation, and enhance UV‑B stress tolerance by upregulating genes for phenylpropanoid, flavonoid, and fatty‑acid pathways. Blue light also promotes epidermal wax accumulation, and ZmCRY1 directly interacts with GLOSSY2 in a light‑dependent manner to drive C32 aldehyde synthesis, linking cryptochrome activity to wax biosynthesis and UV‑B resistance.