Phosphoinositide-specific Phospholipase C 2 (SlPLC2) Facilitates Vesicle Formation and Modulates Immune Signaling in Tomato Phytophthora infestans Interactions
Authors: Perk, E. A., D'Ambrosio, J. M., Cerrudo, I., Robuschi, L., Juarez, M., Velez, P., Mary, V., Theumer, M., Segretin, M. E., Laxalt, A. M.
Date: 2025-09-21 · Version: 1
DOI: 10.1101/2025.09.21.677499 Category: Plant Biology
Model Organism: Solanum lycopersicum
▶ AI Summary
The study demonstrates that the tomato phosphoinositide-specific phospholipase C gene SlPLC2 acts as a susceptibility factor for Phytophthora infestans. CRISPR‑Cas9 knockout of SlPLC2 reduces disease symptoms, pathogen biomass, and early immune responses, while overexpression enhances susceptibility, and SlPLC2 localizes to membranes surrounding infection vesicles. These results link SlPLC2 activity to vesicle formation and hormone signaling during late blight infection.
SlPLC2 Phytophthora infestans susceptibility factor CRISPR-Cas9 knockout plant immunity