The study investigated how Arabidopsis thaliana SR protein kinases (AtSRPKs) regulate alternative RNA splicing by using chemical inhibitors of SRPK activity. Inhibition with SPHINX31 and SRPIN340 caused reduced root growth and loss of root hairs, accompanied by widespread changes in splicing and phosphorylation of genes linked to root development and other cellular processes. Multi‑omics analysis (transcriptomics and phosphoproteomics) revealed that AtSRPKs modulate diverse splicing factors and affect the splicing landscape of numerous pathways.
The study investigates the role of the Arabidopsis transcription factor AtMYB93 in sulfur (S) signaling and root development, revealing that AtMYB93 mutants exhibit altered expression of S transport and metabolism genes and increased shoot S levels, while tomato plants overexpressing SlMYB93 show reduced shoot S. Transcriptomic profiling, elemental analysis, and promoter activity assays indicate that AtMYB93 contributes to root responses to S deprivation, though functional redundancy masks clear phenotypic effects on lateral and adventitious root formation.
The study investigates the Arabidopsis ribosomal protein RPS6A and its role in auxin‑related root growth, revealing that rps6a mutants display shortened primary roots, fewer lateral roots, and defective vasculature that are not rescued by exogenous auxin. Cell biological observations and global transcriptome profiling show weakened auxin signaling and reduced levels of PIN auxin transporters in the mutant, indicating a non‑canonical function of the ribosomal subunit in auxin pathways.
Authors: Anumalla, M., Khanna, A., Catolos, M., Ramos, J., Sta. Cruz, M. T., Venkateshwarlu, C., Konijerla, J., Pradhan, S. K., Dash, S. K., Das, Y., Chowdhury, D., Chetia, S. K., Das, J., Nath, P., Merugumala, G. R., Roy, B., Pradhan, N., Jana, M., Dana, I., Debnath, S., Nath, A., Prasad Singh, S., Iftekharuddaula, K. M., Ghosal, S., Ali, M., Khanam, S., Ul Islam, M. M., Faruquee, M., Tonny, H. J., Hasan, M. R., Rahman, A., Ali, J., Sinha, P., Singh, V., Rafiqul Islam, M., Bhosale, S., Kohli, A., Bhardwaj, H. R., Hussain, W.
The study screened 6,274 elite rice genotypes for submergence and stagnant flooding tolerance, identifying 89 lines with superior performance, including 37 that outperformed SUB1A introgression lines by 40‑50%. These elite lines harbor 86 key QTLs/genes and were used in a novel Transition from Trait to Environment (TTE) breeding strategy, achieving a 65% genetic gain for submergence tolerance and demonstrating strong performance in flood‑prone regions of India and Bangladesh.
A biparental Vicia faba mapping population was screened under glasshouse conditions for resistance to a mixture of Fusarium avenaceum and Fusarium oxysporum, revealing several families with moderate to high resistance. Using the Vfaba_v2 Axiom SNP array, a high-density linkage map of 6,755 SNPs was constructed, enabling the identification of a major QTL on linkage group 4 associated with partial resistance to foot and root rot.
The study profiled the Arabidopsis apoplastic proteome during pattern‑triggered immunity induced by the flg22 peptide, using apoplastic washing fluid with minimal cytoplasmic contamination followed by LC‑MS/MS. Results showed consistent PTI‑specific enrichment and depletion of peptides, a bias toward ectodomain peptides of receptor‑like kinases, and increased abundance of the exosome marker tetraspanin 8, indicating heightened exosome levels during PTI.
A maize near-isogenic line population designed for gene discovery and characterization of allelic effects
Authors: Zhong, T., Mullens, A., Morales, L., Swarts, K., Stafstrom, W., He, Y., Sermons, S., Yang, Q., Lopez-Zuniga, L. O., Rucker, E., Thomason, W., Nelson, R., Jamann, T. M., Balint-Kurti, P., Holland, J. B.
The study characterized 1,264 maize near‑isogenic lines derived from 18 donor inbreds crossed to the recurrent parent B73, using genotyping‑by‑sequencing and SNP‑chip data to detect 2,972 introgression segments via a novel hidden Markov model pipeline. Disease phenotyping enabled QTL mapping for foliar disease resistance, revealing extensive allelic variation among donor lines, and establishing the nNIL population as a valuable resource for dissecting complex traits in maize.