The study generated deep proteome and phosphoproteome datasets from guard cell‑enriched tissue to examine how phosphorylation regulates stomatal movements. Comparative analysis revealed increased phosphorylation of endomembrane trafficking and vacuolar proteins in closed stomata, supporting a role for phospho‑regulated trafficking in stomatal dynamics.

The study used quantitative proteomics and co‑fractionation mass spectrometry to uncover rapid ethylene‑induced changes in protein abundance and complex formation during early seedling development, revealing extensive protein multimerization events that correlate with hypocotyl growth modulation. Small‑scale validation confirmed several identified proteins impact hypocotyl development, highlighting novel components of ethylene‑mediated growth regulation.