Genetius

The study integrates genome, transcriptome, and chromatin accessibility data from 380 soybean accessions to dissect the genetic and regulatory basis of symbiotic nitrogen fixation (SNF). Using GWAS, TWAS, eQTL mapping, and ATAC-seq, the authors identify key loci, co‑expression modules, and regulatory elements, and validate the circadian clock gene GmLHY1b as a negative regulator of nodulation via CRISPR and CUT&Tag. These resources illuminate SNF networks and provide a foundation for soybean improvement.

The chickpea-derived cysteine‑rich peptide NCR13_PFV1 exhibited nanomolar antifungal activity against both QoI‑sensitive and -resistant isolates of the soybean pathogen Cercospora sojina, protecting sprayed soybean leaves without phytotoxicity and showing additive effects with azoxystrobin. The peptide disrupts fungal plasma membranes, induces ROS, is rapidly internalized, binds fungal rRNA, and inhibits protein translation, with iron availability modulating its activity. These multifaceted mechanisms suggest NCR13_PFV1 as a promising bio‑fungicide for durable FLS management.