Genetius

Using a microfluidic valve rootchip, the study simultaneously tracked ROS and calcium dynamics in compressed roots and found three kinetic phases linking mechanosensitive channel activity, NADPH oxidase‑dependent ROS accumulation, and secondary calcium influx. Pharmacological inhibition revealed that a fast calcium response is mediated by plasma‑membrane mechanosensitive channels, while a slower calcium increase is driven by ROS production.

Reduced activity of methylthioadenosine (MTA) nucleosidase causes MTA over‑accumulation in reproductive tissues, leading to lowered cysteine, methionine, and S‑adenosylmethionine levels and altered sulfur and energy metabolism. These metabolic disturbances trigger misregulation of cell‑cycle progression, widespread down‑regulation of developmental genes, and genome‑wide changes in DNA methylation patterns, highlighting the extensive role of MTA recycling in plant growth and methyl‑index maintenance.