Spatiotemporal Analysis Reveals Mechanisms Controlling Reactive Oxygen Species and Calcium Interplay Following Root Compression
Authors: Vinet, P., Audemar, V., Durand-Smet, P., Frachisse, J.-M., Thomine, S.
Date: 2025-10-23 · Version: 1
DOI: 10.1101/2025.10.22.683952 Category: Plant Biology
Model Organism: General
▶ AI Summary
Using a microfluidic valve rootchip, the study simultaneously tracked ROS and calcium dynamics in compressed roots and found three kinetic phases linking mechanosensitive channel activity, NADPH oxidase‑dependent ROS accumulation, and secondary calcium influx. Pharmacological inhibition revealed that a fast calcium response is mediated by plasma‑membrane mechanosensitive channels, while a slower calcium increase is driven by ROS production.
mechanotransduction reactive oxygen species calcium signaling microfluidic compression root biology