Genetius

The study identifies wound‑induced proline transporters ProT2 and ProT3 as central regulators that link salicylic acid signaling to the suppression of de novo root regeneration (DNRR) via modulation of reactive oxygen species dynamics. Genetic loss of these transporters or pharmacological inhibition of proline transport alleviates SA‑mediated regeneration inhibition across several plant species without compromising disease resistance.

The study evaluated the genetically encoded redox biosensor roGFP2-Orp1 for monitoring extracellular redox dynamics in diverse land plants, revealing that re‑oxidation rates in the apoplast differ between Physcomitrium patens and Arabidopsis thaliana and are accelerated by immune activation. Comparisons across tip‑growing cells showed no intracellular redox gradient but a partially reduced extracellular sensor in Nicotiana tabacum pollen tubes, indicating species‑ and cell‑type‑specific oxidative processes.

The study examined five sequential reproductive organ abscission events in two cherry species, revealing that calyx abscission zones form de novo while others are pre‑formed and reactivated by localized ethylene signaling, leading to cell division, differentiation, and lignin deposition. Species‑specific patterns were observed, with P. yedoensis shedding petals independently of pollination and P. sargentii retaining them, and both species using fruit pedicel abscission as a post‑fertilization checkpoint to cull small fruits, indicating a hierarchical reproductive filter.