The study generated the first single‑nucleus RNA‑sequencing dataset of tomato (Solanum lycopersicum) roots colonized by the arbuscular mycorrhizal fungus Rhizophagus irregularis, revealing distinct transcriptional programs in epidermal and cortical cells across stages of arbuscule development. Using unsupervised subclustering and a Motif‑Informed Network Inference (MINI‑EX) approach, the authors identified candidate transcription factors that may coordinate cell‑cycle reactivation and nutrient integration during symbiosis, offering a resource for future functional genetics.
Clathrin-coated vesicles are targeted for selective autophagy during osmotic stress.
Authors: dragwidge, j., Buridan, M., Kraus, J., Kosuth, T., Chambaud, C., Brocard, L., Yperman, K., Mylle, E., Vandorpe, M., Eeckhout, D., De Jaeger, G., Pleskot, R., Bernard, A., Van Damme, D.
The study identifies an autophagy pathway that degrades plasma membrane-derived clathrin-coated vesicles (CCVs) during hyperosmotic stress, helping maintain membrane tension as cell volume decreases. Using live imaging and correlative microscopy, the authors show that the TPLATE complex subunits AtEH1/Pan1 and AtEH2/Pan1 act as selective autophagy receptors by directly binding ATG8, thereby removing excess membrane under drought or salt conditions.