The study profiled root transcriptomes of Arabidopsis wild type and etr1 gain-of-function (etr1-3) and loss-of-function (etr1-7) mutants under ethylene or ACC treatment, identifying 4,522 ethylene‑responsive transcripts, including 553 that depend on ETR1 activity. ETR1‑dependent genes encompassed ethylene biosynthesis enzymes (ACO2, ACO3) and transcription factors, whose expression was further examined in an ein3eil1 background, revealing that both ETR1 and EIN3/EIL1 pathways regulate parts of the network controlling root hair proliferation and lateral root formation.

The study examined how plant‑derived benzoxazinoid metabolites influence interactions among root‑associated bacterial strains and between these bacteria and their plant host. Using both simple pairwise assays and more complex multi‑organism setups, the authors found that these chemicals modulate bacterial‑bacterial and bacterial‑plant interactions, altering plant defense, immunity, and sugar transport especially when bacterial inocula are present. The work highlights the role of the soil chemical legacy in shaping holobiont dynamics and demonstrates the utility of combining reductionist and holistic experimental approaches.

A comparative physiological study of persimmon cultivars with flat (Hiratanenashi) and round (Koushimaru) fruit shapes revealed that differences in cell proliferation, cell shape, and size contribute to shape variation. Principal component analysis of elliptic Fourier descriptors tracked shape changes, while histology and transcriptome profiling identified candidate genes, including a WOX13 homeobox gene, potentially governing fruit shape development.

The study used Arabidopsis thaliana mutants with low (vtc2, vtc4) and high (vtc2/OE-VTC2) ascorbate levels to examine how ascorbate concentration affects gene expression and cellular homeostasis. Transcriptomic analysis revealed that altered ascorbate levels modulate defense and stress pathways, and that TAA1/TAR2‑mediated auxin biosynthesis is required for coping with elevated ascorbate in a light‑dependent manner.

The study investigated how Arabidopsis thaliana SR protein kinases (AtSRPKs) regulate alternative RNA splicing by using chemical inhibitors of SRPK activity. Inhibition with SPHINX31 and SRPIN340 caused reduced root growth and loss of root hairs, accompanied by widespread changes in splicing and phosphorylation of genes linked to root development and other cellular processes. Multi‑omics analysis (transcriptomics and phosphoproteomics) revealed that AtSRPKs modulate diverse splicing factors and affect the splicing landscape of numerous pathways.

The study compares iron deficiency and drought tolerance between two soybean genotypes, Clark (tolerant) and Arisoy (sensitive), using multi‑omics analyses. Clark maintains iron homeostasis, higher antioxidant protein expression, and recruits beneficial root microbes (Variovorax, Paecilomyces) that support nutrient uptake and nodule function, while Arisoy shows impaired physiological and microbial responses. The findings identify host‑microbe interactions and specific molecular pathways as potential targets for breeding and microbiome‑based biofertilizers.

The study investigates the role of the Arabidopsis transcription factor AtMYB93 in sulfur (S) signaling and root development, revealing that AtMYB93 mutants exhibit altered expression of S transport and metabolism genes and increased shoot S levels, while tomato plants overexpressing SlMYB93 show reduced shoot S. Transcriptomic profiling, elemental analysis, and promoter activity assays indicate that AtMYB93 contributes to root responses to S deprivation, though functional redundancy masks clear phenotypic effects on lateral and adventitious root formation.

The study investigates the Arabidopsis ribosomal protein RPS6A and its role in auxin‑related root growth, revealing that rps6a mutants display shortened primary roots, fewer lateral roots, and defective vasculature that are not rescued by exogenous auxin. Cell biological observations and global transcriptome profiling show weakened auxin signaling and reduced levels of PIN auxin transporters in the mutant, indicating a non‑canonical function of the ribosomal subunit in auxin pathways.

The study performed transcriptome profiling of Cryptomeria japonica individuals from different geographic origins grown in three common gardens across Japan, assembling 77,212 transcripts guided by the species' genome. Using SNP-based genetic clustering and weighted gene co‑expression network analysis, they identified gene modules whose expression correlated with genetic differentiation, revealing that defense‑related genes are up‑regulated in Pacific‑side populations while terpenoid metabolism genes are higher in Sea‑of‑Japan populations, indicating local adaptation via regulatory changes.

The study shows that drought triggers ABA accumulation and JA reduction in sorghum roots, accompanied by transcriptional activation of genes linked to mineral homeostasis, hormone signaling, and osmotic regulation, while Fe supplementation enhances ferritin expression and mitigates oxidative stress. Drought also diminishes root bacterial diversity but enriches beneficial taxa such as Burkholderia, whereas fungal diversity remains stable, and functional profiling reveals shifts toward phototrophy, methylotrophy, and nitrate reduction. These findings highlight ferritin’s protective role and suggest specific bacterial inoculants for improving sorghum drought resilience.