The study establishes a tractable system using the large bloom-forming diatom Coscinodiscus granii and its natural oomycete parasite Lagenisma coscinodisci, enabling manual isolation of single host cells and stable co-cultures. High‑quality transcriptomes for both partners were assembled, revealing diverse oomycete effectors and a host transcriptional response involving proteases and exosome pathways, while also profiling the co‑occurring heterotrophic flagellate Pteridomonas sp. This tripartite platform provides a unique marine model for dissecting molecular mechanisms of oomycete‑diatom interactions.

Using a forward genetic screen of 284 Arabidopsis thaliana accessions, the study identified extensive natural variation in root endodermal suberin and pinpointed the previously unknown gene SUBER GENE1 (SBG1) as a key regulator. GWAS and protein interaction analyses revealed that SBG1 controls suberin deposition by binding type‑one protein phosphatases (TOPPs), with disruption of this interaction or TOPP loss‑of‑function altering suberin levels, linking the pathway to ABA signaling.

The study demonstrates that salicylic acid (SA) restricts plant root growth through a mechanism requiring the transmembrane kinase TMK1, which leads to apoplastic alkalinization and inhibition of plasma membrane H⁺-ATPase phosphorylation. This SA effect operates independently of the auxin receptor ABP1, suggesting a novel SA-mediated pathway that balances stress responses with growth.

The study identifies wound‑induced proline transporters ProT2 and ProT3 as central regulators that link salicylic acid signaling to the suppression of de novo root regeneration (DNRR) via modulation of reactive oxygen species dynamics. Genetic loss of these transporters or pharmacological inhibition of proline transport alleviates SA‑mediated regeneration inhibition across several plant species without compromising disease resistance.

The study evaluated a transgenic soybean line (VPZ-34A) expressing Arabidopsis VDE, PsbS, and ZEP for combined improvements in light‑use efficiency and carbon assimilation under ambient and elevated CO2 in a FACE experiment. While VPZ‑34A showed enhanced maximum quantum efficiency of PSII under fluctuating light, it did not increase carbon assimilation efficiency or yield, and transcriptome analysis revealed limited gene expression changes. The results suggest that VPZ‑mediated photosynthetic gains are insufficient to boost productivity under elevated CO2.

The study combined high-throughput image-based phenotyping with genome-wide association studies to uncover the genetic architecture of tolerance to the spittlebug Aeneolamia varia in 339 interspecific Urochloa hybrids. Six robust QTL were identified for plant damage traits, explaining up to 21.5% of variance, and candidate genes linked to hormone signaling, oxidative stress, and cell‑wall modification were highlighted, providing markers for breeding.

The study investigates how the timing of the vegetative phase change (VPC) in Arabidopsis thaliana influences drought adaptation, revealing strong genotype-by-environment interactions that create stage-specific fitness tradeoffs. Genotypes from warmer, drier Iberian climates transition earlier, and genome-wide association mapping identifies loci linked to VPC timing and drought response, with several candidates validated using T‑DNA insertion lines.

The study investigates the role of the chromatin regulator MpSWI3, a core subunit of the SWI/SNF complex, in the liverwort Marchantia polymorpha. A promoter mutation disrupts male gametangiophore development and spermiogenesis, causing enhanced vegetative propagation, and transcriptomic analysis reveals that MpSWI3 regulates genes controlling reproductive initiation, sperm function, and asexual reproduction, highlighting its ancient epigenetic role in balancing vegetative and reproductive phases.

The study examined drought tolerance across nine provenances of the conifer Pinus nigra using high‑throughput phenotyping combined with metabolomic and transcriptomic analyses under controlled soil‑drying conditions. Drought tolerance, measured by the decline in Fv/Fm, varied among provenances but was not linked to a climatic gradient and was independent of growth, with tolerant provenances showing distinct flavonoid and diterpene profiles and provenance‑specific gene expression patterns. Integrating phenotypic and molecular data revealed metabolic signatures underlying drought adaptation in this non‑model conifer.

The study created transgenic Arabidopsis lines enabling inducible plasmodesmal closure via an overactive CALLOSE SYNTHASE3 allele (icals3m) and the C‑terminal domain of PDLP1, independent of pathogen signals. Induced closure triggered stress‑responsive gene expression, elevated salicylic acid levels, and enhanced resistance to Pseudomonas syringae, while also causing starch accumulation, reduced growth, and increased susceptibility to Botrytis cinerea, indicating that plasmodesmal closure itself can activate immune signaling.