Genetius

The study identifies wound‑induced proline transporters ProT2 and ProT3 as central regulators that link salicylic acid signaling to the suppression of de novo root regeneration (DNRR) via modulation of reactive oxygen species dynamics. Genetic loss of these transporters or pharmacological inhibition of proline transport alleviates SA‑mediated regeneration inhibition across several plant species without compromising disease resistance.

The study engineered autoluminescent reporter constructs that visually indicate jasmonic and salicylic acid signaling with up to 53‑fold contrast. Using consumer‑grade cameras, the authors monitored hormone activity in Arabidopsis thaliana and Nicotiana benthamiana throughout development and during pest and pathogen attacks.