Phenotypic scoring of Canola Blackleg severity using machine learning image analysis
Authors: Hu, Q., Anderson, S. N., Gardner, S., Ernst, T. W., Koscielny, C. B., Bahia, N. S., Johnson, C. G., Jarvis, A. C., Hynek, J., Coles, N., Falak, I., Charne, D. R., Ruidiaz, M. E., Linares, J. N., Mazis, A., Stanton, D. J.
The study introduces a deep‑learning based image analysis pipeline that scores blackleg disease severity from stem cross‑section images of canola species, achieving greater consistency than median expert raters while preserving comparable heritability of susceptibility traits. This standardized scoring method aims to improve selection of resistant varieties in breeding programs.
The study presents a deep‑learning pipeline that uses state‑of‑the‑art convolutional neural networks to automatically estimate the establishment of perennial groundcovers in agricultural research plots from smartphone images. By employing region‑of‑interest markers and deploying the models on AWS SageMaker with a lightweight Django web interface, the approach provides fast, objective, and reproducible assessments that can be adopted by researchers and growers across the Midwest.
Unraveling the cis-regulatory code controlling abscisic acid-dependent gene expression in Arabidopsis using deep learning
Authors: Opdebeeck, H., Smet, D., Thierens, S., Minne, M., De Beukelaer, H., Zuallaert, J., Van Bel, M., Van Montagu, M., Degroeve, S., De Rybel, B., Vandepoele, K.
The study used an interpretable convolutional neural network to predict ABA responsiveness from proximal promoter sequences in Arabidopsis thaliana, revealing both known ABF-binding motifs and novel regulatory elements. Model performance was boosted by advanced data augmentation, and predicted regulatory regions were experimentally validated using reporter lines, confirming the inferred cis‑regulatory code.
A comparative physiological study of persimmon cultivars with flat (Hiratanenashi) and round (Koushimaru) fruit shapes revealed that differences in cell proliferation, cell shape, and size contribute to shape variation. Principal component analysis of elliptic Fourier descriptors tracked shape changes, while histology and transcriptome profiling identified candidate genes, including a WOX13 homeobox gene, potentially governing fruit shape development.
The study presents GenoRetriever, an interpretable deep learning framework trained on STRIPE-seq data from soybean and other crops, that predicts transcription start site locations and usage by identifying 27 core promoter motifs. Validation using in silico motif insertions, saturation mutagenesis, and CRISPR‑Cas9 promoter editing demonstrates high predictive accuracy and reveals domestication‑driven motif usage shifts and lineage‑specific effects. The tool is provided via a web server for promoter analysis and design, offering a new resource for plant functional genomics and crop improvement.
The Global Wheat Full Semantic Organ Segmentation (GWFSS) dataset
Authors: Wang, Z., Zenkl, R., Greche, L., De Solan, B., Bernigaud Samatan, L., Ouahid, S., Visioni, A., Robles-Zazueta, C. A., Pinto, F., Perez-Olivera, I., Reynolds, M. P., Zhu, C., Liu, S., D'argaignon, M.-P., Lopez-Lozano, R., Weiss, M., Marzougui, A., Roth, L., Dandrifosse, S., Carlier, A., Dumont, B., Mercatoris, B., Fernandez, J., Chapman, S., Najafian, K., Stavness, I., Wang, H., Guo, W., Virlet, N., Hawkesford, M., Chen, Z., David, E., Gillet, J., Irfan, K., Comar, A., Hund, A.
The Global Wheat Dataset Consortium released a comprehensive semantic segmentation dataset (GWFSS) of wheat organs across developmental stages, comprising 1,096 fully annotated images and 52,078 unannotated images from 11 institutions. Models based on DeepLabV3Plus and Segformer were trained, with Segformer achieving ≈90% mIoU for leaves and spikes but lower precision (54%) for stems, while also enabling weed exclusion and discrimination of necrotic, senescent, and residue tissues.
The study investigates the role of the Arabidopsis transcription factor AtMYB93 in sulfur (S) signaling and root development, revealing that AtMYB93 mutants exhibit altered expression of S transport and metabolism genes and increased shoot S levels, while tomato plants overexpressing SlMYB93 show reduced shoot S. Transcriptomic profiling, elemental analysis, and promoter activity assays indicate that AtMYB93 contributes to root responses to S deprivation, though functional redundancy masks clear phenotypic effects on lateral and adventitious root formation.
The study introduces an in-soil fiber Bragg grating (FBG) sensing system that continuously records three-dimensional strain from growing pseudo-roots, enabling non‑destructive monitoring of root architecture. Using two ResNet models, the system predicts root width and depth with over 90% accuracy, and performance improves to 96‑98% after retraining on data from actual corn (Zea mays) roots over a 30‑day period. This prototype demonstrates potential for scalable, real‑time root phenotyping and broader soil environment sensing.
The study performed transcriptome profiling of Cryptomeria japonica individuals from different geographic origins grown in three common gardens across Japan, assembling 77,212 transcripts guided by the species' genome. Using SNP-based genetic clustering and weighted gene co‑expression network analysis, they identified gene modules whose expression correlated with genetic differentiation, revealing that defense‑related genes are up‑regulated in Pacific‑side populations while terpenoid metabolism genes are higher in Sea‑of‑Japan populations, indicating local adaptation via regulatory changes.