The study investigates the subfunctionalization of duplicated GIGANTEA paralogs in Petunia x hybrida, focusing on PhGI2's role in floral transition. While RNAi silencing of PhGI2 caused mixed vegetative and reproductive phenotypes, CRISPR/Cas9-generated loss‑of‑function alleles showed that PhGI2 specifically promotes flowering without affecting vegetative growth or scent. The findings reveal that PhGI2 is essential for maintaining circadian gene expression dynamics and regulating flowering time, separating vegetative growth from reproductive timing.
CLPC2 plays specific roles in CLP complex-mediated regulation of growth, photosynthesis, embryogenesis and response to growth-promoting microbial compounds
Authors: Leal-Lopez, J., Bahaji, A., De Diego, N., Tarkowski, P., Baroja-Fernandez, E., Munoz, F. J., Almagro, G., Perez, C. E., Bastidas-Parrado, L. A., Loperfido, D., Caporalli, E., Ezquer, I., Lopez-Serrano, L., Ferez-Gomez, A., Coca-Ruiz, V., Pulido, P., Morcillo, R. J. L., Pozueta-Romero, J.
The study demonstrates that the plastid chaperone CLPC2, but not its paralogue CLPC1, is essential for Arabidopsis responsiveness to microbial volatile compounds and for normal seed and seedling development. Loss of CLPC2 alters the chloroplast proteome, affecting proteins linked to growth, photosynthesis, and embryogenesis, while overexpression of CLPC2 mimics CLPC1 deficiency, highlighting distinct functional roles within the CLP protease complex.