Regenerative agriculture effects on biomass, drought resilience and 14C-photosynthate allocation in wheat drilled into ley compared to disc or ploughed arable soil
Authors: Austen, N., Short, E., Tille, S., Johnson, I., Summers, R., Cameron, D. D., Leake, J. R.
Regenerative agriculture using a grass-clover ley increased wheat yields and macroaggregate stability despite reduced root biomass, but did not enhance soil carbon sequestration as measured by 14C retention. Drought further decreased photosynthate allocation to roots, especially in ley soils, while genotype effects on yield were minimal.
The study isolated an endophytic Pseudomonas aeruginosa strain (SPT08) from tomato cotyledon seedlings that suppressed the wilt pathogen Ralstonia pseudosolanacearum and promoted plant growth, increasing height by 20% and root biomass by 60%. GFP labeling confirmed endophytic colonization, and genomic analysis revealed multiple secretion systems and secondary‑metabolite gene clusters associated with biocontrol and growth‑promoting traits.
The study examined how soil phosphorus and nitrogen availability influence wheat root-associated arbuscular mycorrhizal fungal (AMF) communities and the expression of mycorrhizal nutrient transporters. Field sampling across two years combined with controlled pot experiments showed that P and N jointly affect AMF colonisation, community composition (with Funneliformis dominance under high P), and regulation of phosphate, ammonium, and nitrate transporters. Integrating metabarcoding and RT‑qPCR provides a framework to assess AMF contributions to crop nutrition.
The study compared aphid resistance and Barley Yellow Dwarf Virus (BYDV) transmission among three wheat varieties (G1, RGT Wolverine, RGT Illustrious). G1 emits the repellent 2‑tridecanone, restricts aphid phloem access, and shows reduced BYDV transmission, whereas RGT Wolverine limits systemic viral infection despite high transmission efficiency. The authors suggest breeding the two resistance mechanisms together for improved protection.
Gain and loss of gene function shaped the nickel hyperaccumulation trait in Noccaea caerulescens
Authors: Belloeil, C., Garcia de la Torre, V. S., Contreras Aguilera, R., Kupper, H., Lopez-Roques, C., Iampetro, C., Vandecasteele, C., Klopp, C., Launay-Avon, A., Leemhuis, W., Yamjabok, J., van den Heuvel, J., Aarts, M. G. M., Quintela Sabaris, C., Thomine, S., MERLOT, S.
The study presents a high-quality genome assembly for the nickel hyperaccumulator Noccaea caerulescens and uses it as a reference for comparative transcriptomic analyses across different N. caerulescens accessions and the non‑accumulating relative Microthlaspi perfoliatum. It identifies a limited set of metal transporters (NcHMA3, NcHMA4, NcIREG2, and NcIRT1) whose elevated expression correlates with hyperaccumulation, and demonstrates that frameshift mutations in NcIRT1 can abolish the trait, indicating an ancient, transporter‑driven origin of nickel hyperaccumulation.
Introducing furanocoumarin biosynthetic genes in tomato results in coumarins accumulation and impacted growth
Authors: Bouille, A., Villard, C., Galati, G., Roumani, M., Fauvet, A., Grosjean, J., Hoengenaert, L., Boerjan, W., Ralph, J., Hilliou, F., Robin, C., Hehn, A., Larbat, R.
The study engineered the linear furanocoumarin pathway in tomato by integrating four biosynthetic genes, aiming to produce psoralen, but instead generated coumarins such as scopoletin. Morphophysiological, metabolomic, and transcriptomic analyses revealed that even low levels of these coumarins can influence plant growth and physiology, highlighting both benefits and costs of coumarin accumulation in crops.
The study investigates hormetic responses of tomato (Solanum lycopersicum) seedlings to low‑dose cadmium, demonstrating enhanced growth through morphological, biochemical, and histochemical analyses. Transcriptomic profiling revealed differential expression of oxidoreductase genes, signaling components, and several long non‑coding RNAs (lncRNAs) that generate miRNAs (sly‑MIR396a and sly‑MIR1063g), which modulate target genes to promote growth. In‑silico analyses of lncRNA targets and miRNA precursors provide mechanistic insight into cadmium‑induced hormesis and its potential for crop improvement.
The study investigated whether wheat homoeologous genes actively compensate for each other when one copy acquires a premature termination codon (PTC) mutation. By analyzing mutagenised wheat lines, the authors found that only about 3% of cases exhibited upregulation of the unaffected homoeolog, indicating that widespread active transcriptional compensation is absent in wheat.
Tomato leaf transcriptomic changes promoted by long-term water scarcity stress can be largely prevented by a fungal-based biostimulant
Authors: Lopez-Serrano, L., Ferez-Gomez, A., Romero-Aranda, R., Jaime Fernandez, E., Leal Lopez, J., Fernandez Baroja, E., Almagro, G., Dolezal, K., Novak, O., Diaz, L., Bautista, R., Leon Morcillo, R. J., Pozueta Romero, J.
Foliar application of Trichoderma harzianum cell‑free culture filtrates (CF) increased fruit yield, root growth, and photosynthesis in a commercial tomato cultivar under prolonged water deficit in a Mediterranean greenhouse. Integrated physiological, metabolite, and transcriptomic analyses revealed that CF mitigated drought‑induced changes, suppressing about half of water‑stress responsive genes, thereby reducing the plant’s transcriptional sensitivity to water scarcity.
MdBRC1 and MdFT2 Interaction Fine-Tunes Bud Break Regulation in Apple
Authors: Gioppato, H. A., Estevan, J., Al Bolbol, M., Soriano, A., Garighan, J., Jeong, K., Georget, C., Soto, D. G., El Khoury, S., Falavigna, V. d. S., George, S., Perales, M., Andres, F.
The study identifies the transcription factor MdBRC1 as a key inhibitor of bud growth during the ecodormancy phase in apple (Malus domestica), directly regulating dormancy‑associated genes and interacting with the flowering promoter MdFT2 to modulate bud break. Comparative transcriptomic analysis and gain‑of‑function experiments in poplar demonstrate that MdFT2 physically binds MdBRC1, attenuating its repressive activity and acting as a molecular switch for the transition to active growth.