The study identifies the circadian clock component ELF3 as a temporal gatekeeper that limits hormone‑induced pericycle proliferation and lateral root development in Arabidopsis thaliana. Time‑resolved transcriptomics, imaging, and genetic analyses show that ELF3 maintains rhythmic expression of key regulators via LNK1 and MADS‑box genes, and that loss of ELF3 disrupts this rhythm, enhancing callus growth and accelerating root organogenesis.

The study reveals that the thermosensor and circadian regulator ELF3 interacts with the PLT3 transcription factor in Arabidopsis root stem cell niches, forming subcellular condensates that sustain quiescent centre and columella stem cell fate. ELF3’s intrinsically disordered prion‑like domains drive condensate formation with PLT3, and PIF3/4 act as nuclear shuttles recruiting ELF3 to nuclear condensates, linking environmental cues to stem cell maintenance.

The study examined how DNA methylation influences cold stress priming in Arabidopsis thaliana, revealing that primed plants exhibit distinct gene expression and methylation patterns compared to non-primed plants. DNA methylation mutants, especially met1 lacking CG methylation, showed altered cold memory and misregulation of the CBF gene cluster, indicating that methylation ensures transcriptional precision during stress recall.

The authors compiled and standardized published data on Rubisco dark inhibition for 157 flowering plant species, categorizing them into four inhibition levels and analyzing phylogenetic trends. Their meta‑analysis reveals a complex, uneven distribution of inhibition across taxa, suggesting underlying chloroplast microenvironment drivers and providing a new resource for future photosynthesis improvement efforts.

The study examines how ectopic accumulation of methionine in Arabidopsis thaliana leaves, driven by a deregulated AtCGS transgene under a seed‑specific promoter, reshapes metabolism, gene expression, and DNA methylation. High‑methionine lines exhibit increased amino acids and sugars, activation of stress‑hormone pathways, and reduced expression of DNA methyltransferases, while low‑methionine lines show heightened non‑CG methylation without major transcriptional changes. Integrated transcriptomic and methylomic analyses reveal a feedback loop linking sulfur‑carbon metabolism, stress adaptation, and epigenetic regulation.

The study investigates how cortical microtubule orientations are coordinated across tissues, testing the hypothesis that they sense mechanical stress. By quantifying microtubule behavior on different cell faces and edges, the authors find that tissue-level biases arise from cell‑geometric factors and edge‑specific mechanisms rather than direct stress sensing, and they develop a combinatorial model incorporating face‑specific and edge‑specific microtubule dynamics to explain the observed coordination.

The study isolated the Plant Cysteine Oxidase/Ethylene Response Factor VII oxygen‑sensing circuit from Arabidopsis thaliana and reconstituted it in Saccharomyces cerevisiae, using a reporter to compare hypoxia‑induced transcriptional dynamics in yeast and plants. Both systems showed rapid ERFVII stabilization, but plants exhibited a larger response, which could be enhanced in yeast by adding a hypoxia‑inducible feedback loop. Computational modeling identified promoter competition and hypoxia‑inducible PCOs as key determinants of early hypoxia responses.

The study shows that the SnRK1 catalytic subunit KIN10 directs tissue-specific growth‑defense programs in Arabidopsis thaliana by reshaping transcriptomes. kin10 knockout mutants exhibit altered root transcription, reduced root growth, and weakened defense against Pseudomonas syringae, whereas KIN10 overexpression activates shoot defense pathways, increasing ROS and salicylic acid signaling at the cost of growth.

A forward genetic screen in light-grown Arabidopsis seedlings identified the Evening Complex component ELF3 as a key inhibitor of phototropic hypocotyl bending under high red:far-red and blue light, acting upstream of PIF4/PIF5. ELF3 and its partner LUX also mediate circadian regulation of phototropism, and the orthologous ELF3 in Brachypodium distachyon influences phototropism in the opposite direction.

The study investigates the altered timing of the core circadian oscillator gene ELF3 in wheat compared to Arabidopsis, revealing that dawn-specific expression in wheat arises from repression by TOC1. An optimized computational model integrating experimental expression data and promoter architecture predicts that wheat’s circadian oscillator remains robust despite this shift, indicating flexibility in plant circadian network design.