Phosphite (Phi) and phosphate (Pi) share the same root uptake system, but Phi acts as a biostimulant that modulates plant growth and disease resistance in a species‑ and Pi‑dependent manner. In Arabidopsis, Phi induces hypersensitive‑like cell death and enhances resistance to Plectosphaerella cucumerina, while in rice it counteracts Pi‑induced susceptibility to Magnaporthe oryzae and Fusarium fujikuroi, accompanied by extensive transcriptional reprogramming.
The study developed a molecular assay to identify Myrtle rust‑resistant Melaleuca quinquenervia individuals for restoration. Artificial inoculations were followed by whole‑genome sequencing of 492 seedlings and a GWAS that pinpointed SNP clusters in three chromosomal regions, including candidate R‑genes, which were refined into a 1,049‑SNP panel achieving a genomic prediction accuracy of R = 0.83.
An Axiom SNP genotyping array for potato: development, evaluation and applications
Authors: Baig, N., Thelen, K., Ayenan, M. A. T., Hartje, S., Obeng-Hinneh, E., Zgadzaj, R., Renner, J., Muders, K., Truberg, B., Rosen, A., Prigge, V., Bruckmueller, J., Luebeck, J., Van Inghelandt, D., Stich, B.
The study reports the creation and validation of a high‑density Axiom SNP array for Solanum tuberosum, based on 10X Genomics sequencing of 108 diverse clones and integration of existing Illumina markers. The array demonstrated high reproducibility and, after filtering, provided 206,616 informative markers for population structure analysis, GWAS of polyphenol oxidase activity, and genomic prediction with accuracies up to 0.86.
The study integrated weekly morphophysiological measurements with high-density genotyping-by-sequencing data and a machine‑learning pipeline to dissect flowering time variation in diverse Cannabis sativa landraces. By applying mutual information, recursive feature elimination, random forest, and support vector machine classifiers to over 234,000 combined genetic, phenotypic, and environmental features, the authors identified 53 key markers that classify early, medium, and late flowering types with 96.6% accuracy. Notable loci, including CsFT3 and CsCFL1, were highlighted as promising targets for breeding and smart‑crop strategies.
Whole genome sequencing-based multi-locus association mapping for kernel iron, zinc and protein content in groundnut
Authors: Sagar, U. N., Parmar, S., Gangurde, S. S., Sharma, V., Pandey, A. K., Mohinuddin, D. K., Dube, N., Bhat, R. S., John, K., Sreevalli, M. D., Rani, P. S., Singh, K., Varshney, R. K., Pandey, M. K.
The study used multi‑season phenotyping for iron, zinc, and protein content together with whole‑genome re‑sequencing of a groundnut mini‑core collection to conduct a genome‑wide association study, identifying numerous marker‑trait associations and candidate genes linked to nutrient homeostasis. SNP‑based KASP markers were designed for nine loci, of which three showed polymorphism and are ready for deployment in genomics‑assisted breeding for nutrient‑rich groundnut varieties.
The study examines how the SnRK1 catalytic subunit KIN10 integrates carbon availability with root growth regulation in Arabidopsis thaliana. Loss of KIN10 reduces glucose‑induced inhibition of root elongation and triggers widespread transcriptional reprogramming of metabolic and hormonal pathways, notably affecting auxin and jasmonate signaling under sucrose supplementation. These findings highlight KIN10 as a central hub linking energy status to developmental and environmental cues in roots.
The study identified a major QTL (qDTH3) on chromosome 3 responsible for a 7‑10‑day earlier heading phenotype in the rice line SM93, using QTL‑seq, KASP genotyping, association mapping, and transcriptomic analysis to fine‑map the locus to a 2.53 Mb region and pinpoint candidate genes. SNP markers linked to these genes were proposed as tools for breeding early‑maturing, climate‑resilient rice varieties.