The study tracked molecular changes in plastoglobules and thylakoids of Zea mays B73 during heat stress and recovery, revealing increased plastoglobule size, number, and adjacent lipid droplets over time. Proteomic and lipidomic analyses uncovered up‑regulation of specific plastoglobule proteins and alterations in triacylglycerol, plastoquinone derivatives, and phytol esters, suggesting roles in membrane remodeling and oxidative defense. These insights highlight plastoglobule‑associated pathways as potential targets for enhancing heat resilience in maize.

The study applied the STOmics spatial transcriptomics platform to map gene expression at subcellular resolution in developing wheat (Triticum aestivum) seeds during grain filling, analyzing over four million transcripts. Eight functional cellular groups were identified, including four distinct endosperm clusters with radial expression patterns and novel marker genes, and subgenome‑biased expression was observed among specific paralogs. These results highlight spatial transcriptomics as a powerful tool for uncovering tissue‑specific and polyploid‑specific gene regulation in seeds.

The study combined spatial transcriptomics and single-nuclei RNA sequencing to map soybean (Glycine max) responses to Asian soybean rust caused by Phakopsora pachyrhizi, revealing two distinct host cell states: pathogen‑occupied regions and adjacent non‑infected regions that show heightened defense gene expression. Gene co‑expression network analysis identified a key immune‑related module active in the stressed cells, highlighting a cell‑non‑autonomous defense mechanism.

The study uses an imputation strategy that integrates deep single-cell RNA sequencing with spatial gene expression data to map transcriptional dynamics across barley inflorescence development at cellular resolution. By leveraging the BARVISTA web interface, the authors identify key transcriptional events in meristem founder cells, characterize complex branching mutants, and reconstruct spatio‑temporal trajectories of flower organogenesis, offering insights for targeted trait manipulation.

The study identifies the serine/threonine protein kinase CIPK14/SNRK3.15 as a regulator of sulfate‑deficiency responses in Arabidopsis thaliana seedlings, with mutants showing diminished early adaptive and later salvage responses under sulfur starvation. While snrk3.15 mutants exhibit no obvious phenotype under sufficient sulfur, the work provides a novel proteomic dataset comparing wild‑type and mutant seedlings under sulfur limitation.

The study examines how the SnRK1 catalytic subunit KIN10 integrates carbon availability with root growth regulation in Arabidopsis thaliana. Loss of KIN10 reduces glucose‑induced inhibition of root elongation and triggers widespread transcriptional reprogramming of metabolic and hormonal pathways, notably affecting auxin and jasmonate signaling under sucrose supplementation. These findings highlight KIN10 as a central hub linking energy status to developmental and environmental cues in roots.

The study examined how white lupin (Lupinus albus) cotyledons mobilize nitrogen and minerals during early seedling growth under nitrogen‑deficient conditions, revealing that 60 % of stored proteins degrade within eight days and are redirected to support development. Proteomic analyses showed dynamic shifts in nutrient transport, amino acid metabolism, and stress responses, and premature cotyledon removal markedly impaired growth, highlighting the cotyledon's essential role in nutrient supply and transient photosynthetic activity.

The study characterizes the protein composition of extracellular vesicles (EVs) secreted by the oomycete Phytophthora infestans, revealing enrichment of transmembrane proteins and RxLR effectors, while EV-independent secretions are dominated by cell wall–modifying enzymes. Two MARVEL‑domain proteins, PiMDP1 and PiMDP2, are identified as EV-associated markers that co‑localize with RxLR effectors, with PiMDP2 specifically accumulating at the haustorial interface during early infection, suggesting a role in effector delivery.

The study provides an in‑depth proteomic characterization of brewer's spent grain (BSG) and tracks proteome dynamics during malting and mashing, revealing that 29% of identified proteins change in abundance and that B3‑Hordein dominates the BSG protein pool. BSG contains a high proportion of intracellular proteins and over 45% of its proteins are potential allergens or antinutritional factors, underscoring the need for targeted downstream processing to create safe, functional food ingredients.

The study investigated how Arabidopsis thaliana SR protein kinases (AtSRPKs) regulate alternative RNA splicing by using chemical inhibitors of SRPK activity. Inhibition with SPHINX31 and SRPIN340 caused reduced root growth and loss of root hairs, accompanied by widespread changes in splicing and phosphorylation of genes linked to root development and other cellular processes. Multi‑omics analysis (transcriptomics and phosphoproteomics) revealed that AtSRPKs modulate diverse splicing factors and affect the splicing landscape of numerous pathways.