Phosphite (Phi) and phosphate (Pi) share the same root uptake system, but Phi acts as a biostimulant that modulates plant growth and disease resistance in a species‑ and Pi‑dependent manner. In Arabidopsis, Phi induces hypersensitive‑like cell death and enhances resistance to Plectosphaerella cucumerina, while in rice it counteracts Pi‑induced susceptibility to Magnaporthe oryzae and Fusarium fujikuroi, accompanied by extensive transcriptional reprogramming.
The study created a system that blocks root‑mediated signaling between wheat varieties in a varietal mixture and used transcriptomic and metabolomic profiling to reveal that root chemical interactions drive reduced susceptibility to Septoria tritici blotch, with phenolic compounds emerging as key mediators. Disruption of these root signals eliminates both the disease resistance phenotype and the associated molecular reprogramming.
The study investigated the ability of foliar-applied salicylic acid (SA) to alleviate drought stress in the high‑altitude medicinal plant Valeriana wallichii by measuring physiological and biochemical responses during vegetative and flowering stages. SA at specific concentrations improved photosynthetic rates, water‑use efficiency, chlorophyll content, membrane stability, and root biomass under both severe (25% field capacity) and moderate (50% field capacity) drought conditions. These results suggest that SA treatment enhances drought tolerance and productivity in this species.
The study employed high‑resolution micro‑CT and machine‑learning analysis to compare three‑dimensional leaf anatomy of wild‑type sorghum and a transgenic line expressing a synthetic Epidermal Patterning Factor (EPFsyn) that reduces stomatal density. Despite the lower stomatal density, overall leaf internal CO₂ conductance (gias) was unchanged because EPFsyn plants displayed larger sub‑stomatal crypts and altered mesophyll airspace, indicating a compensatory anatomical response. These results illuminate the coordination between surface stomatal patterning and internal mesophyll structure in a C4 species, informing water‑use efficiency engineering.
Field trials in Tamil Nadu evaluated how irrigation method, fertiliser regimen, and rice variety affect methane emissions, water use, and yield. Drip irrigation cut seasonal methane emissions by up to 68% and water use by 42.5% with minimal yield loss, while a tailored fertiliser package further reduced emissions by 28% under flood conditions. Varietal differences also influenced emissions, highlighting the potential of integrated agronomic practices for climate‑friendly rice production.
The study presents an optimized Agrobacterium-mediated transformation protocol for bread wheat that incorporates a GRF4‑GIF1 fusion to enhance regeneration and achieve genotype‑independent transformation across multiple cultivars. The approach consistently improves transformation efficiency while limiting pleiotropic effects, offering a versatile platform for functional genomics and gene editing in wheat.
The study examined how limited water (60–65% field capacity) during the vegetative stage affects stomatal and non‑stomatal leaf traits linked to water‑use efficiency in Australian elite rice varieties. While limited water reduced photosynthetic efficiency, stomatal conductance, and stomatal density, it increased cuticular and epicuticular wax deposition, conserving water without compromising grain yield, milling recovery, or most seed quality attributes.
The study examines how the SnRK1 catalytic subunit KIN10 integrates carbon availability with root growth regulation in Arabidopsis thaliana. Loss of KIN10 reduces glucose‑induced inhibition of root elongation and triggers widespread transcriptional reprogramming of metabolic and hormonal pathways, notably affecting auxin and jasmonate signaling under sucrose supplementation. These findings highlight KIN10 as a central hub linking energy status to developmental and environmental cues in roots.
A spontaneous mutation in a key C4 pathway gene significantly alters leaf δ13C uncoupling its relationship with WUE and photosynthetic performance in Zea mays
Authors: Twohey, R. J., Crawford, J. D., Runyon, M. M., Xie, J., Leakey, A. D. B., Cousins, A. B., Studer, A. J.
The study used a Zea mays line with an exceptionally negative δ13C value to uncover a large deletion in the carbonic anhydrase1 gene via quantitative genetics. Contrary to C4 isotope theory, the mutant allele showed increased carbonic anhydrase activity and reduced δ13C, indicating a decoupling of δ13C from intrinsic water-use efficiency and highlighting the need for further investigation of carbon isotope discrimination in C4 crops.