Genetius

Parallel quantitative proteome and transcriptome time‑course profiling of Arabidopsis thaliana revealed that circadian clock genes, especially the morning‑expressed LHY/CCA1 module, exert extensive control over diel proteome rhythmicity, far exceeding their impact on the transcriptome. This control creates a bimodal phase distribution of rhythmic proteins that is lost in clock‑deficient mutants, indicating pervasive post‑translational regulation of gene expression by the circadian system.

The study investigates how division plane orientation is established in cambium stem cells (CSCs) of Arabidopsis thaliana, revealing that orientation is independent of spindle positioning and the preprophase band (PPB) but relies on the cortical division zone (CDZ) and POK kinesins. Using microtubule imaging and PPB-deficient mutants, the authors demonstrate that CSCs consistently divide along their longest axis through CDZ-mediated mechanisms.

The study examined how increased stomatal density and transpiration in the Arabidopsis thaliana epf1 epf2 double mutant affect root hydraulic properties, finding that despite higher water loss the mutant maintains leaf water status and displays reduced root hydraulic conductivity (Lpr) without changes in aquaporin expression. Under PEG‑induced osmotic stress, the wild type showed lower Lpr than the mutant, suggesting that elevated xylem tension acts as a long‑distance signal coordinating reductions in both stomatal aperture and root water transport.

The study examined how DNA methylation influences cold stress priming in Arabidopsis thaliana, revealing that primed plants exhibit distinct gene expression and methylation patterns compared to non-primed plants. DNA methylation mutants, especially met1 lacking CG methylation, showed altered cold memory and misregulation of the CBF gene cluster, indicating that methylation ensures transcriptional precision during stress recall.

The study expressed a Brachypodium phenylalanine/tyrosine ammonia‑lyase (PTAL) in Arabidopsis thaliana to create plants that initiate phenylpropanoid biosynthesis from phenylalanine, tyrosine, or both. While the engineered pathway did not affect growth in wild‑type plants, tyrosine‑specific initiation rescued the lethal phenotype of c4h mutants but caused developmental defects due to accumulation of cis‑cinnamic acid, highlighting the evolutionary importance of the canonical phenylalanine‑derived route.

The study characterizes the phytolongin PHYL2.1, a longin domain protein lacking a SNARE motif, localized to the endoplasmic reticulum in Arabidopsis thaliana. Loss‑of‑function mutants (Atphyl2.1‑1 and Atphyl2.1‑2) display reduced root cell division, altered meristem architecture, and defective cell plate formation involving the SNARE KNOLLE, leading to shorter roots.

Using a forward genetic screen of 284 Arabidopsis thaliana accessions, the study identified extensive natural variation in root endodermal suberin and pinpointed the previously unknown gene SUBER GENE1 (SBG1) as a key regulator. GWAS and protein interaction analyses revealed that SBG1 controls suberin deposition by binding type‑one protein phosphatases (TOPPs), with disruption of this interaction or TOPP loss‑of‑function altering suberin levels, linking the pathway to ABA signaling.

The study investigates the role of the bZIP transcription factor HY5 in Arabidopsis thaliana’s tolerance to combined high‑light and heat stress (HLHS). HY5 overexpression enhances photosynthetic efficiency, reduces membrane damage, and improves leaf health under HLHS, while HY5 deficiency leads to hypersensitivity, linked to altered accumulation of photosystem II proteins, impaired non‑photochemical quenching via NPQ4/PsbS, and disrupted ABA and JA signaling. Proteomic and hormonal analyses reveal HY5 as a central regulator coordinating photoprotective proteins and hormone networks under multifactorial stress.

The study demonstrates that ELF4 is essential for recruiting ELF3 into hypocotyl nuclei at dusk, a process that enhances ELF3’s ability to repress target gene expression and limit hypocotyl elongation, especially under short‑day conditions. Subnuclear localization patterns of ELF3 differ between hypocotyl and root tissues, indicating tissue‑specific temporal regulation by ELF4.

The study examined how Turnip mosaic virus (TuMV) infection reshapes root-associated bacterial and fungal communities in two Arabidopsis thaliana genotypes. TuMV markedly reduced bacterial diversity and altered community composition in a genotype‑specific manner, while fungal communities stayed stable; bacterial co‑occurrence networks later recovered and even increased in complexity, highlighting microbial resilience. These findings underscore virus‑driven selective filtering of bacterial root microbiota and the role of host genotype in mediating microbiome responses to viral stress.