The study examined how dual‑purpose hemp (Cannabis sativa) adjusts to different phosphate levels, showing that flower biomass is maintained unless phosphate is completely removed. Integrated physiological measurements and transcriptomic profiling revealed that phosphate is reallocated to flowers via glycolytic bypasses and organic phosphate release, while key regulatory genes followed expected patterns but did not suppress uptake at high phosphate, leading to nitrate depletion that limits growth.

The study identified a heat‑responsive exon‑skipping event in the basic Helix‑Loop‑Helix domain of the transcription factor PIF4, which reduces PIF4 activity and promotes photomorphogenic traits in etiolated seedlings. This reveals a novel post‑transcriptional mechanism by which plants modulate PIF4 function during heat stress.

The study characterizes the plant spliceosomal protein AtSF1 in Arabidopsis thaliana, using iCLIP and RNA‑seq to map its in vivo branch point binding sites and demonstrate that loss of AtSF1 causes widespread 3' splice‑site mis‑selection. Structural comparison reveals a plant‑specific domain architecture, and the identified AtSF1 targets are enriched for circadian and defense genes, linking splicing regulation to timing and immunity.

The study used untargeted metabolomics and QTL mapping in a tomato recombinant inbred line population to characterize root exudate composition and identify genetic loci controlling specific metabolites. It reveals domestication-driven changes in exudate profiles and links metabolic QTLs with previously reported microbial QTLs, suggesting a genetic basis for shaping the root microbiome.

Using ten Phaeodactylum tricornutum mutant strains with graded constitutive Lhcx1 expression, the study links NPQ induction under high light to physiological outcomes (oxidized QA, increased cyclic electron flow) and extensive transcriptomic reprogramming, affecting nearly half the genome. The approach demonstrates that higher NPQ mitigates PSII damage, boosts ATP production for repair, and drives distinct gene regulatory networks, providing a model framework for dissecting photosynthetic and gene expression integration.

The study establishes a tractable system using the large bloom-forming diatom Coscinodiscus granii and its natural oomycete parasite Lagenisma coscinodisci, enabling manual isolation of single host cells and stable co-cultures. High‑quality transcriptomes for both partners were assembled, revealing diverse oomycete effectors and a host transcriptional response involving proteases and exosome pathways, while also profiling the co‑occurring heterotrophic flagellate Pteridomonas sp. This tripartite platform provides a unique marine model for dissecting molecular mechanisms of oomycete‑diatom interactions.

The study identifies the serine/arginine-rich splicing factor RS2Z36 as a key regulator of ovary patterning and early fruit morphology in tomato, with loss‑of‑function mutants producing smaller, ellipsoid fruits and elongated pericarp cells. RNA‑seq and proteomic analyses reveal widespread alternative splicing and altered protein abundance, including novel splice‑variant peptides, while mutant pericarps show increased deposition of LM6‑detected arabinan and AGP epitopes.

The study functionally characterizes a conserved structured RNA motif (45ABC) in Arabidopsis RBP45 pre‑mRNAs, showing that its sequence and pairing elements mediate a negative auto‑ and cross‑regulatory feedback loop through alternative splicing that produces unproductive isoforms and reduces RBP45 expression. Transcriptome‑wide splicing analysis and phenotypic assessment of rbp45 mutants reveal that RBP45B plays a dominant role and that proper regulation of this motif is essential for root growth and flowering time.

The study evaluated a transgenic soybean line (VPZ-34A) expressing Arabidopsis VDE, PsbS, and ZEP for combined improvements in light‑use efficiency and carbon assimilation under ambient and elevated CO2 in a FACE experiment. While VPZ‑34A showed enhanced maximum quantum efficiency of PSII under fluctuating light, it did not increase carbon assimilation efficiency or yield, and transcriptome analysis revealed limited gene expression changes. The results suggest that VPZ‑mediated photosynthetic gains are insufficient to boost productivity under elevated CO2.

The study investigates the molecular basis of heat‑tolerant pollen tube growth in tomato (Solanum lycopersicum) by comparing thermotolerant and sensitive cultivars. Using live imaging, transcriptomics, proteomics, and genetics, the authors identified the Rapid Alkalinization Factor (RALF) signaling pathway as a key regulator of pollen tube integrity under high temperature, with loss of a specific RALF peptide enhancing tube integrity in a thermotolerant cultivar.