Genetius

The study characterizes the chloroplast‑localized protein AT4G33780 in Arabidopsis thaliana using CRISPR/Cas9 knockout and overexpression lines, revealing tissue‑specific expression and context‑dependent effects on seed germination, seedling growth, vegetative development, and root responses to nickel stress. Integrated transcriptomic (RNA‑seq) and untargeted metabolomic analyses show extensive transcriptional reprogramming—especially of cell‑wall genes—and altered central energy metabolism, indicating AT4G33780 coordinates metabolic state with developmental regulation rather than controlling single pathways.

The authors performed a progressive, sublethal drought experiment in Arabidopsis thaliana, integrating large‑scale phenotyping with time‑resolved transcriptomics and machine‑learning analysis. They identified distinct drought stages governed by multiple, largely gradual transcriptional programs that overlap with the plant aging program, and found that transcripts strongly predictive of phenotypes serve as biomarkers rather than causal drivers.

Zinc deficiency remodels the Arabidopsis root tip by reducing RAM size while preserving meristem activity and local Zn levels, leading to enhanced cell elongation and differentiation. Transcriptomic analysis revealed a unique Zn‑deficiency response in the RAM-enriched zone, with ZIP12 being the most strongly induced gene, and zip12 mutants displayed severe defects in root growth, RAM architecture, Zn‑responsive gene expression, and metal distribution. These results highlight ZIP12 as a key regulator of Zn homeostasis and root meristem function under micronutrient stress.

The study investigates how miR394 influences flowering time in Arabidopsis thaliana by combining transcriptomic profiling of mir394a mir394b double mutants with histological analysis of reporter lines. Bioinformatic analysis identified a novel lncRNA overlapping MIR394B (named MIRAST), and differential promoter activity of MIR394A and MIR394B suggests miR394 fine‑tunes flower development through transcription factor and chromatin remodeler regulation.