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HSP90-mediated stress resilience in male gametophyte of Arabidopsis thaliana

Authors: Kahrizi, Z., Michailidis, C., Raabe, K., Fila, J., Klodova, B., Rudolf, J., Prochazkova Schrumpfova, P., Honys, D., Fragkostefanakis, S.

Date: 2025-06-23 · Version: 1
DOI: 10.1101/2025.06.21.660849

Category: Plant Biology

Model Organism: General

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The study performed stage‑specific transcriptomic profiling of pollen development under normal and heat stress conditions, revealing that most heat‑responsive genes are unique to each developmental stage, with the bi‑cellular stage showing the greatest number of differentially expressed genes. Promoter analysis indicated stage‑specific enrichment of heat shock elements, and knockdown of HSP90 via RNAi reduced pollen germination and caused nuclear defects, especially under heat stress, linking HSP90 to ABA signaling and DNA metabolism during pollen development.

heat shock proteins pollen development heat stress transcriptomics HSP90 RNAi