The study engineered plastid-targeted genetically encoded biosensors to visualize several phosphoinositide species (PI3P, PI4P, PI5P, PI(4,5)P2, PI(3,5)P2) within chloroplasts and confirmed their specificity via immunological assays. Co‑expression with PPI‑modifying enzymes revealed altered biosensor distribution, an association between PI3P and the chloroplast protein VIPP1, and that elevated PPI levels under stress change localization and increase drought sensitivity, highlighting PPIs in plant stress responses.