The study used ft1 knock‑out mutants in barley to demonstrate that the florigen homolog FT1 links vegetative and reproductive meristem development with plant metabolism, influencing source‑sink dynamics, longevity, and fertility. Loss of FT1 caused altered leaf and inflorescence determinacy, increased organ size but reduced fertility, and a transcriptional shift toward photosynthetic and carbon‑catabolism genes, while soluble sugar and starch accumulated in inflorescences, indicating diminished sink strength.
The study investigates the wheat Pm3 NLR allelic series, revealing that near-identical Pm3d and Pm3e alleles confer broad-spectrum resistance by recognizing multiple, structurally diverse powdery mildew effectors. Using chimeric NLR constructs, the authors pinpoint specificity-determining polymorphisms and demonstrate that engineered combinations of Pm3d and Pm3e further expand effector recognition, showcasing the potential for durable wheat protection through NLR engineering.
The study generated comprehensive hormonal profiles of hazelnut catkins across the dormancy period, revealing that abscisic acid declines while gibberellins rise, resulting in a decreasing ABA/GA ratio that correlates with dormancy release. Cytokinins unexpectedly increased throughout dormancy, auxin conjugates and ethylene precursors showed distinct patterns between early and late blooming accessions, suggesting hormone manipulation could delay bloom.
Evaluation of combined root exudate and rhizosphere microbiota sampling approaches to elucidate plant-soil-microbe interaction
Authors: Escudero-Martinez, C., Browne, E. Y., Schwalm, H., Santangeli, M., Brown, M., Brown, L., Roberts, D. M., Duff, A. M., Morris, J., Hedley, P. E., Thorpe, P., Abbott, J. C., Brennan, F., Bulgarelli, D., George, T. S., Oburger, E.
The study benchmarked several sampling approaches for simultaneous profiling of root exudates and rhizosphere microbiota in soil-grown barley, revealing consistent exudate chemistry across methods but variation in root morphology and nitrogen exudation. High‑throughput amplicon sequencing and quantitative PCR showed protocol‑specific impacts on microbial composition, yet most rhizosphere-enriched microbes were captured by all approaches. The authors conclude that different protocols provide comparable integrated data, though methodological differences must be aligned with experimental objectives.
The study demonstrates that limonene, a natural essential‑oil component, strongly inhibits Fusarium oxysporum, the causal agent of potato dry rot, by impairing colony growth, hyphal morphology, spore viability, membrane integrity, and transcription/translation processes, as well as disrupting ion homeostasis. Combined treatments reveal additive effects with mancozeb and synergistic effects with hymexazol, highlighting limonene's potential as an eco‑friendly bio‑fungicide for potato disease management.
Regenerative agriculture effects on biomass, drought resilience and 14C-photosynthate allocation in wheat drilled into ley compared to disc or ploughed arable soil
Authors: Austen, N., Short, E., Tille, S., Johnson, I., Summers, R., Cameron, D. D., Leake, J. R.
Regenerative agriculture using a grass-clover ley increased wheat yields and macroaggregate stability despite reduced root biomass, but did not enhance soil carbon sequestration as measured by 14C retention. Drought further decreased photosynthate allocation to roots, especially in ley soils, while genotype effects on yield were minimal.
The study examined how allelic variation at three barley flowering-time genes (PPD‑H1, ELF3, and PHYC) influences photoperiod response parameters, revealing that ELF3 reduces intrinsic earliness and PhyC‑e lowers photoperiod sensitivity. By testing Near Isogenic Lines and HEB‑25 lines under 16–24 h photoperiods, the authors identified a 20‑h threshold for PPD‑H1 lines and proposed reduced photoperiod regimes (20 h and 16 h) for energy‑efficient speed breeding.
The study examined how soil phosphorus and nitrogen availability influence wheat root-associated arbuscular mycorrhizal fungal (AMF) communities and the expression of mycorrhizal nutrient transporters. Field sampling across two years combined with controlled pot experiments showed that P and N jointly affect AMF colonisation, community composition (with Funneliformis dominance under high P), and regulation of phosphate, ammonium, and nitrate transporters. Integrating metabarcoding and RT‑qPCR provides a framework to assess AMF contributions to crop nutrition.
The study compared aphid resistance and Barley Yellow Dwarf Virus (BYDV) transmission among three wheat varieties (G1, RGT Wolverine, RGT Illustrious). G1 emits the repellent 2‑tridecanone, restricts aphid phloem access, and shows reduced BYDV transmission, whereas RGT Wolverine limits systemic viral infection despite high transmission efficiency. The authors suggest breeding the two resistance mechanisms together for improved protection.
The study profiled small interfering RNAs (siRNAs) in barley (Hordeum vulgare) seeds differing in viability after controlled long‑term storage, identifying 85,728 differentially expressed siRNAs associated with seed vigor. Trans‑acting siRNAs displayed distinct temporal patterns during imbibition, and functional analyses linked siRNA targets to key processes such as cytochrome activity, root development, and carbohydrate metabolism, suggesting a role in maintaining metabolic activity during germination.