Regenerative agriculture effects on biomass, drought resilience and 14C-photosynthate allocation in wheat drilled into ley compared to disc or ploughed arable soil
Authors: Austen, N., Short, E., Tille, S., Johnson, I., Summers, R., Cameron, D. D., Leake, J. R.
Regenerative agriculture using a grass-clover ley increased wheat yields and macroaggregate stability despite reduced root biomass, but did not enhance soil carbon sequestration as measured by 14C retention. Drought further decreased photosynthate allocation to roots, especially in ley soils, while genotype effects on yield were minimal.
The study examined how soil phosphorus and nitrogen availability influence wheat root-associated arbuscular mycorrhizal fungal (AMF) communities and the expression of mycorrhizal nutrient transporters. Field sampling across two years combined with controlled pot experiments showed that P and N jointly affect AMF colonisation, community composition (with Funneliformis dominance under high P), and regulation of phosphate, ammonium, and nitrate transporters. Integrating metabarcoding and RT‑qPCR provides a framework to assess AMF contributions to crop nutrition.
The study investigated structural cell wall changes in wheat (Triticum aestivum) seedlings during drought, revealing rapid remodeling after five days, organ-specific responses, and both cross‑linking and degradation of wall polymers such as homogalacturonans, xylan, and AGPs. Deposition of unesterified homogalacturonans promotes calcium cross‑linking, enhancing wall rigidity and water retention.
The study compared aphid resistance and Barley Yellow Dwarf Virus (BYDV) transmission among three wheat varieties (G1, RGT Wolverine, RGT Illustrious). G1 emits the repellent 2‑tridecanone, restricts aphid phloem access, and shows reduced BYDV transmission, whereas RGT Wolverine limits systemic viral infection despite high transmission efficiency. The authors suggest breeding the two resistance mechanisms together for improved protection.
Gain and loss of gene function shaped the nickel hyperaccumulation trait in Noccaea caerulescens
Authors: Belloeil, C., Garcia de la Torre, V. S., Contreras Aguilera, R., Kupper, H., Lopez-Roques, C., Iampetro, C., Vandecasteele, C., Klopp, C., Launay-Avon, A., Leemhuis, W., Yamjabok, J., van den Heuvel, J., Aarts, M. G. M., Quintela Sabaris, C., Thomine, S., MERLOT, S.
The study presents a high-quality genome assembly for the nickel hyperaccumulator Noccaea caerulescens and uses it as a reference for comparative transcriptomic analyses across different N. caerulescens accessions and the non‑accumulating relative Microthlaspi perfoliatum. It identifies a limited set of metal transporters (NcHMA3, NcHMA4, NcIREG2, and NcIRT1) whose elevated expression correlates with hyperaccumulation, and demonstrates that frameshift mutations in NcIRT1 can abolish the trait, indicating an ancient, transporter‑driven origin of nickel hyperaccumulation.
The study used a yeast two-hybrid screen to identify 52 wheat proteins that interact with the inositol pyrophosphate kinase TaVIH2-3B, highlighting the fasciclin‑like arabinogalactan protein TaFLA7 as a key partner involved in cell‑wall functions. Pulldown assays and reporter fusion analyses confirmed the interaction and plasma‑membrane localization of TaFLA7, which is modulated by TaVIH2‑3B activity and shows drought‑responsive and grain‑development expression in wheat.
The study investigated whether wheat homoeologous genes actively compensate for each other when one copy acquires a premature termination codon (PTC) mutation. By analyzing mutagenised wheat lines, the authors found that only about 3% of cases exhibited upregulation of the unaffected homoeolog, indicating that widespread active transcriptional compensation is absent in wheat.
MdBRC1 and MdFT2 Interaction Fine-Tunes Bud Break Regulation in Apple
Authors: Gioppato, H. A., Estevan, J., Al Bolbol, M., Soriano, A., Garighan, J., Jeong, K., Georget, C., Soto, D. G., El Khoury, S., Falavigna, V. d. S., George, S., Perales, M., Andres, F.
The study identifies the transcription factor MdBRC1 as a key inhibitor of bud growth during the ecodormancy phase in apple (Malus domestica), directly regulating dormancy‑associated genes and interacting with the flowering promoter MdFT2 to modulate bud break. Comparative transcriptomic analysis and gain‑of‑function experiments in poplar demonstrate that MdFT2 physically binds MdBRC1, attenuating its repressive activity and acting as a molecular switch for the transition to active growth.
Cell layer-specific modification of cell wall is associated with exo-mesocarp split in pistachio (Pistacia vera L.)
Authors: Zhang, S., Wang, M., Eagle, S., Chernikova, A., Bedell, K. M., Tran, P., Lee, C., Adaskaveg, J. A., Wei, Y., Lopez, R., Basco, A., Gordon, P., Blanco-Ulate, B., Monroe, G., Drakakaki, G.
The study characterizes the anatomical layers of the pistachio hull and demonstrates that layer‑specific cell wall modifications, particularly pectin-related gene expression changes, drive cell expansion, loss of adhesion, and hull splitting during late fruit development. Combined transcriptomic profiling, immunohistochemistry, and anatomical analyses reveal that filler parenchyma expands while hypodermal parenchyma remains static, and field observations link irrigation and humidity to hull split, highlighting the interplay of molecular, cellular, and environmental factors.
Overexpression of the wheat bHLH transcription factor TaPGS1 leads to increased flavonol accumulation in the seed coat, which disrupts polar auxin transport and causes localized auxin accumulation, delaying endosperm cellularization and increasing cell number, thereby enlarging grain size. Integrated metabolomic and transcriptomic analyses identified upregulated flavonol biosynthetic genes, revealing a regulatory module that links flavonol-mediated auxin distribution to seed development in wheat.