Genetius

The study employed deep learning-based image restoration to obtain high‑resolution 4D fluorescence images of actin dynamics during the early stages of cell plate formation in transgenic tobacco BY‑2 cells. Using Lifeact‑RFP and RFP‑ABD2 actin probes, the authors discovered distinct localization patterns, with Lifeact‑RFP marking actin both near the daughter nucleus and around the nascent cell plate, suggesting a functional role in plate initiation.

Flavodiiron proteins (FLVs) from the moss Physcomitrium patens were stably expressed in Nicotiana tabacum, resulting in transgenic lines with markedly higher pseudo‑cyclic electron transport (PCET) rates. Under sudden light increases, FLV activity competed with cyclic electron transport, alleviating PSI acceptor‑side limitation and enhancing protection of the photosynthetic apparatus. These results demonstrate that FLVs can function effectively in an angiosperm crop under fluctuating light conditions.

The study investigated the nanodomain localization of tobacco PIN auxin transporters (NtPIN11T, NtPIN2T, NtPIN3bT) in BY-2 cultured cells, revealing that each PIN occupies specific plasma‑membrane domains and that NtPIN11T is the most uniformly distributed and least mobile, with its immobilization depending on the actin and microtubule cytoskeleton and cell‑wall components. Pharmacological perturbations, high‑resolution microscopy, immunoelectron microscopy, and co‑immunoprecipitation identified novel interaction partners, notably GPI‑anchored arabinogalactan proteins, suggesting that cell‑wall‑mediated immobilization modulates PIN function.