Genetius

The study demonstrates that the plasmodesmata‑located protein PDLP5 negatively regulates plasma membrane intrinsic protein (PIP)‑mediated hydrogen peroxide transport in Arabidopsis by forming extracellular complexes with PIPs, notably PIP2;5. Overexpression of PDLP5 reduces H2O2 uptake and mitigates H2O2‑induced root growth inhibition, while loss‑of‑function mutants show increased sensitivity, revealing a novel extracellular mechanism of aquaporin regulation.

The study examined intraspecific variation and thermal sensitivity of residual leaf conductance (g_res) in silver fir (Abies alba), assessing genetic differences among French provenances and environmental plasticity across forest sites. While genetic differences were minimal, g_res showed strong seasonal and site-dependent plasticity linked to cuticular wax accumulation, whereas thermal parameters and xylem embolism vulnerability (P50) remained relatively stable. These findings suggest g_res is a developmentally dynamic trait that could enhance model predictions of tree drought vulnerability under warming climates.

The study reports a chromosome-scale genome of the Epichloe host grass Achnatherum inebrians and identifies conserved cell pluripotency regulators (ARF5/7/19, BBM, WUS/WOX5, CUC1/2) as predictors of a narrow temporal window for callus regeneration, enabling a 49.4 % transformation efficiency. Applying this CPR-based timing strategy to wheat and sainfoin increased shoot regeneration up to 87.5 %, demonstrating its broader applicability.

The study used transcriptomics to compare tomato's compatible responses to three evolutionarily distant pathogens—nematodes, aphids, and oomycetes—identifying differentially expressed genes and key host hubs involved in parasitism. Co‑expression and GO enrichment analyses revealed shared dysregulated clusters, and translational mapping onto the Arabidopsis interactome highlighted central tomato candidates for multi‑pathogen control strategies.

The study characterized cabbage stem flea beetle (CSFB) larval development and assessed antibiosis across a diverse Brassica panel, finding no larval resistance within Brassica napus but consistent antibiosis in Sinapis alba. It also demonstrated that Brassica rapa and Arabidopsis thaliana can serve as suitable hosts for CSFB, enabling future genetic studies of resistance. The results suggest using Brassica relatives to explore CSFB resistance genetics for breeding oilseed rape.

The study enhances virus-induced genome editing (VIGE) by introducing a multiplexed guide RNA system and an engineered high‑activity Ymu1 variant (Ymu1‑WFR) delivered via Tobacco Rattle Virus, enabling efficient, transgene‑free, and heritable multiplex editing. This approach builds on prior transgene‑free VIGE in Arabidopsis using ISYmu1 TnpB and expands its utility for single‑locus and multiplex genome modifications.

The study identifies a cysteine‑rich peptide, FEM, secreted by Arabidopsis thaliana stylar tissues that blocks heterospecific pollen tube elongation, functioning independently of the known barrier component SPRI1. A neighboring pollen‑expressed protein, HOM, interacts with FEM to neutralize its effect, forming a toxin‑antidote system that contributes to multilayered prezygotic reproductive isolation.

The study compared heat stress responses in diploid (T. monococcum), tetraploid (T. turgidum), and hexaploid (T. aestivum) wheat, finding that the tetraploid retained the highest grain yield under severe heat while the hexaploid showed the most extensive transcriptional changes. Transcriptome and splicing analyses revealed ploidy‑dependent regulatory reprogramming, including a heat‑induced exon‑skipping event in a NF‑YB transcription factor unique to hexaploid wheat, highlighting distinct strategies for heat tolerance across wheat ploidy levels.

The study introduced an in‑situ system with 24 cuvettes to continuously monitor leaf gas exchange and VOC emissions on mature Fagus sylvatica and Pseudotsuga menziesii in a temperate forest, alongside sap flux, radial growth, and tree water deficit measurements. Results revealed species‑specific responses to midsummer drying, with reduced stomatal conductance and VOC emissions in sun‑exposed P. menziesii branches, while F. sylvatica sun leaves increased gas exchange, and highlighted time lags between leaf and stem water fluxes.

The study demonstrates that silica‑dried plant material can be effectively used for flow‑cytometric genome size and ploidy estimation across a large flora, analyzing ~45,000 samples from 1,135 Eastern Alpine angiosperm species. Using a mostly universal protocol, they obtained ploidy data for 97% of the species, revealing that 34% contain polyploid cytotypes and 16% are ploidy‑variable, and they provide a comprehensive dataset for the botanical community.